Otto Antoine was a member of the Association of Berlin Artists, which was founded in 1841. Later, he represented his fellow artists as a member of the association’s board and was repeatedly involved in the setting up of the Greater Berlin Art Exhibition. Eventually, he became an honorary member and honorary president of the association.
Immediately after moving from Koblenz to Berlin in 1891, Antoine contacted local artists in Berlin. He studied part-time at the Academy of Fine Arts (Hochschule für Bildende Künste) and attended courses in landscape and nude painting.